How to make AA

[Right Hook]

Acetic Acid is becoming very hard to find these days...but thought this was helpful:

The calculation:
Distilled white vinegar is supposed to be standardized to ~5% acetic acid, which would make it 850mM. To get it to the recommended 100mM, you'd want 11.76% white vinegar (100mM/850mM = 11.76%). Since it would be almost impossible to draw out 11.76IU's, I round this to 12, which is certainly going to be close to our desired 100mM.


The filtering process:
I use off the shelf grocery store distilled white vinegar. In order to ensure safety, I filter it using .20u whatman filters. Here is the step by step for those that may not be familiar with filtering using whatmans. What you will want to have on hand before starting out is some sterile vials, some .20u whatman filters, some syringes and needles (I use a 10cc syringe, and .23 gauge 1" needles), and some alcohol swabs.


(1) First draw up about 10cc of the distilled white vinegar
(2) screw on the .20u whatman to the 10cc syringe (or whatever size you use)
(3) screw on a .23 gauge needle (or whatever size you decide to use)
(4) take your sterile vial, swab the top with alcohol, insert a needle for venting.
(5) Insert your syringe/whatman/needle apparatus and slowly push the 10cc's into the sterile vial.


Now you have safe vinegar to use for your reconstituting.


ALTERNATE METHOD - Alternately, you could simply mix your water and distilled white vinegar before filtering using about 7.5 parts of water per 1 part of distilled white vinegar. After mixing these together in this ratio, run the mixture through your .20u whatman as above. You will end up with a vial of dilutent this way that has the proper PH for use with your IGF-1.


Reconstituting:


How much water/vinegar you reconstitute with is going to somewhat depend on which LR3 IGF-1 you are using. Igtropin is shipped in 100mcg vials, which I usually reconstitute at 1ml(cc) per 100mcg vial (which will make the 10 mark on your insulin syringe = 10 mcgs). The gropep based IGF-1's are primarily shipped in 1mg vials, and I usually use 5ml for these (which will make the 10 mark on your insulin syringe = 20mcgs).
At any rate, what I do is:


(1) take an alcohol swab and swab the tops of my water, vinegar solution, and IGF-1 vials
(2) take a syringe with a 23 gauge, 1" needle and draw out .12 cc's of vinegar for the 100mcg vials or .60 cc's for the 1mg vials.
(3) next I take this syringe and draw out the water - .88cc's for 100mcg, 4.4cc's for the 1mg.


FOR ALTERNATE METHOD in lieu of steps (2) and (3) - Just draw out the desired amount of dilutent from your pre-mixed vial of
vinegar / water.


(4) next i poke the needle into the LR3 IGF-1 vial and dribble this solution down the side of the vial, avoid any direct spray on the lyophilized powder until all of the dilutent is in the vial
(5) using a gentle swirling motion, I reconstitute the powder.
(6) I stick the vial in the fridge and it is now ready for use.


Well, I think that about sums it up. Hope this helps anyone who may have been wondering about using vinegar to reconstitute. I would advise that if you end up using Igtropin, you seriously consider using this vinegar method. Igtropin and other dilutents such as BA do not get along well together at all.


RedBaron

[sfearl1]

this one sinner! lol

[thesinner]

Vinegar is typically 5-8% acetic acid. If I remember correctly, Grunt76 recommends 0.5%. I am assuming this is either weight% or %v/v.

With the amounts we're using the differences in densities between AA and water are negligable. So we can assume wt% = %v/v

I got some stuff I gotta take care of right now. pKa of acetic acid is 4.8. We can test for pH, but I gotta look some stuff up as to correlate. pH of your finished AA should be about 4.5

7-9 parts water per one part vinegar should be sufficient.